Peculiarities of <i>Hypericum perforatum</i> L. reproduction <i>in vitro</i> culture and development of plants in the open field

The possibility of using the method cell and tissue culture for microclonal reproduction St. Johns wort is shown. Direct morphogenesis was obtained seedlings without roots at age 12 days. It found that introduction BAP in a low concentration (0,1 mg/l) with IAA 1,0 mg/l into MS nutrient medium promoted minimum bud formation maximum yield morphologically normal shoots need an elongation phase. At stage rhizogenesis, process root observed all variants experiment; proportion high ranged from 88 to 90%. height shoots, number pairs leaves per shoot, length plant were reached on mg/l. period adaptation non-sterile conditions 30 days, while regenerant plants observed. survival rate adapted field (100%). Regenerant developed rapidly bloomed first year. revealed before planting open ground, propagated vitro passed containers soil mixture formed sufficiently system large adventitious 68 cm long. base 2 5 renewal 912 formed. On 65th day after transplanting, more than doubled averaged 7,8 pcs. individual shoot 23 cm. In third decade August, entered budding phase, September 20, they flowering noted by beginning flowering, increased 4954 cm, diameter up 0,4 From 16 24 oblong-ovoid standard plant. floral part (inflorescence) 2027 long began axils 914 consisted 710 lateral axes (paracladia). average flowers 82. amplitude variability main morphological traits studied levels. development microclonally end growing season corresponded growth second year life grown seedlings.